Quantitative evaluation of the uncertainty in spatial topological relations can provide the reliable basis for applications in automatic evaluation of multi-scale topological relation consistency, spatial reasoning, and spatial queries. A geometric measure-based topological distance was defined. A rough set model for uncertainty in topological relation proposed to explore a quantitative method for determining topological distance. Furthermore, this paper presents the measure index for uncertainty in spatial topological relations of multi-scale spatial entities. A case study shows that the model proposed in this paper is reasonable, and suitable for the quantitative evaluation of topological relations found in multi-scale representation processes.