Abstract: From the perspective of individual differences, we studied the influence of gender, reference system and map-browsing style on the time and path selection in 3D virtual scene. The spatial 3D scene of the Palace Museum was chosen as the experimental environment. The perspective of participates was limited as the first view while they completed the tasks in 3D virtual scene to simulate the process of wayfinding in daily life. The experimental results show that different genders had a significant impact on the time, and wayfinding time for male was significantly smaller than that for female, but the path complexity of the subjects completed the wayfinding in the 3D scene had no gender differences. The completion time of three reference systems was obviously different, and those people who selected the coordination reference system took the shortest time, but there was no difference in the aspect of path complexity. The effect of different map-browsing styles on path complexity was significant, and the overall map-browsing type for wayfinding in 3D scene was better than the landmark map-browsing type.