Abstract: The influence of GPS precise clock and observation data sampling internal on LEO kinematic and reduced-dynamic precise orbit determination (POD) was analyzed. The several experiments of LEO POD were carried out respectively by using the 30 s or 5 s sampling interval GPS precise clock from CODE and the 30 s or 10 s GPS observation data. The results show that 5 s GPS clock and 10 s observation data comparing with 30 s clock and 30 s observation data, the accuracy of reduced-dynamic POD is improved by 16%, and the kinematic POD is 8.8%. The accuracy of LEO POD is the worst by using 10 s observation data and 30 s GPS clock. For 30 s observation data, the accuracy of LEO POD by using 30 s GPS clock is consistent with 5 s GPS clock products from CODE.