Abstract: The most important causes of horizontal reference stations are human factors such as excessive pumping of groundwater and immoderate mineral exploitation. GPS data from Anhui Operating Reference Stations(AHCORS) between 2011-11 and 2013-9 was continuously processed the using GAMIT/GLOBK software. Then, based on these results, the 3D velocities of the reference stations were determined, and crustal horizontal movement and land vertical movement was analyzed in detail. It was found that the mean horizontal velocity of AHCORS reference stations was 34.44 mm/a in the direction of E18.01°S under ITRF2008 reference frame and 6.13 mm/a in the direction of E7.31°S with regard to the Eurasia Plate. Stations located in Huaibei Plain have moved in horizontal with 1~4 mm/a relative to No-Net-Rotation of the whole AHCORS. Tancheng-Lujiang Fault showed sinistral strike slip feature between Jiashan and Sixian, with very weak movement in the south of Jiashan. As for the vertical direction, AHCORS reference stations uplift in the south of the Huai River, but subside in the north of it. The average uplift rate of the stations located in the south of the Huai River is 2.72 mm/a, while the average subside rate of the stations located in the north of the Huai River is 2.98 mm/a.